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primary antibodies against occludin  (Proteintech)


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    Structured Review

    Proteintech primary antibodies against occludin
    Primary Antibodies Against Occludin, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1559 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+occludin/Occludin+Antibody/pm41813664-271-3-26
    Average 96 stars, based on 1559 article reviews
    primary antibodies against occludin - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Western Blot:

    Article Title: Biological valorization of methane and nitrogen gas-derived ammonia via methanotrophic bacteria for gut-beneficial nutrients.
    Article Snippet: .. For western blotting, primary antibodies against Occludin (Cat No. 27260-1-AP, dilution 1: 35000) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, Cat No. 10494-1-AP, dilution 1: 30000) were purchased from Proteintech Group, Inc. .. The second antibody (Cat No. A21020 dilution 1: 20000) was purchased from Abbkine Scientific Co., Ltd.47,48 The relative expression of Occludin was normalized by GAPDH.

    other:

    Article Title: Molecular hydrogen supplementation in mice ameliorates lipopolysaccharide‐induced loss of interest
    Article Snippet: After electrophoresis, membranes were washed in PBS with 0.5% Tween 20, then transferred into 5% Blocking One blocking solution (Nacalai Tesque) for 1 h. Membranes were probed with primary antibodies against GFAP (ab53554, 1:10,000, Abcam), IBA1 (ab5076, 1:200, Abcam), PBR (ab109497, 1:200, Abcam), occludin (13409‐1‐AP, 1:2,000, ProteinTech), ZO‐1 (Ab221547, 1:2,000, Abcam), and α‐tubulin (1:50,000, FUJIFILM Wako Pure Chemical Corporation) at 4°C overnight.

    Article Title: House dust mite induced mucosal barrier dysfunction and type 2 inflammatory responses via the MAPK/AP-1/IL-24 Signaling pathway in allergic rhinitis.
    Article Snippet: Both cells and tissue sections were incubated overnight at 4 ◦C with primary antibodies against ZO-1 (1:100, Proteintech), occludin (1:100, Proteintech), and MBP (1:100, ABclonal).

    Blocking Assay:

    Article Title: Periplanta americana extract regulates the Th17/Treg cell balance via Notch1 in ulcerative colitis
    Article Snippet: The equal amount of protein was separated with sodium dodecyl sulfate -polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Merck Millipore, LG18-1501M-1H). .. After blocking with 5% skim milk for 1.5 h (h) at room temperature, the membranes were incubated overnight at 4°C with different primary antibodies against Occludin (13409-1-AP, Proteintech), Claudin 1 (13050-1-A, Proteintech), ZO1 (TA5145F, Abmart; 21773-1-A, Proteintech), Foxp3 (Ab215206, Abcam), RORγt (13205-1-AP, Proteintech), Math1 (21215-1-A, Proteintech), Notch1 (T55244S, Abmart; 20687-1-AP, Proteintech), Hes1(11988S, CST) and β-actin (ab179467; Abcam). .. Followed by secondary antibodies for 1 h. The PVDF membranes were detected using a chemiluminescence imager (Bio-Rad) and quantified using the Image Lab software (Version 4.1, Bio-Rad).

    Article Title: Periplanta americana extract regulates the Th17/Treg cell balance via Notch1 in ulcerative colitis.
    Article Snippet: The equal amount of protein was separated with sodium dodecyl sulfate -polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Merck Millipore, LG181501M-1H). .. After blocking with 5% skim milk for 1.5 h (h) at room temperature, the membranes were incubated overnight at 4°C with different primary antibodies against Occludin (13409-1- AP, Proteintech), Claudin 1 (13050-1-A, Proteintech), ZO1 (TA5145F, Abmart; 21773-1-A, Proteintech), Foxp3 (Ab215206, Abcam), RORγt (13205-1-AP, Proteintech), Math1 (21215-1-A, Proteintech), Notch1 (T55244S, Abmart; 20687-1-AP, Proteintech), Hes1(11988S, CST) and β-actin (ab179467; Abcam). .. Followed by secondary antibodies for 1 h. The PVDF membranes were detected using a chemiluminescence imager (Bio-Rad) and quantified using the Image Lab software (Version 4.1, Bio-Rad).

    Incubation:

    Article Title: Periplanta americana extract regulates the Th17/Treg cell balance via Notch1 in ulcerative colitis
    Article Snippet: The equal amount of protein was separated with sodium dodecyl sulfate -polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Merck Millipore, LG18-1501M-1H). .. After blocking with 5% skim milk for 1.5 h (h) at room temperature, the membranes were incubated overnight at 4°C with different primary antibodies against Occludin (13409-1-AP, Proteintech), Claudin 1 (13050-1-A, Proteintech), ZO1 (TA5145F, Abmart; 21773-1-A, Proteintech), Foxp3 (Ab215206, Abcam), RORγt (13205-1-AP, Proteintech), Math1 (21215-1-A, Proteintech), Notch1 (T55244S, Abmart; 20687-1-AP, Proteintech), Hes1(11988S, CST) and β-actin (ab179467; Abcam). .. Followed by secondary antibodies for 1 h. The PVDF membranes were detected using a chemiluminescence imager (Bio-Rad) and quantified using the Image Lab software (Version 4.1, Bio-Rad).

    Article Title: Periplanta americana extract regulates the Th17/Treg cell balance via Notch1 in ulcerative colitis.
    Article Snippet: The equal amount of protein was separated with sodium dodecyl sulfate -polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Merck Millipore, LG181501M-1H). .. After blocking with 5% skim milk for 1.5 h (h) at room temperature, the membranes were incubated overnight at 4°C with different primary antibodies against Occludin (13409-1- AP, Proteintech), Claudin 1 (13050-1-A, Proteintech), ZO1 (TA5145F, Abmart; 21773-1-A, Proteintech), Foxp3 (Ab215206, Abcam), RORγt (13205-1-AP, Proteintech), Math1 (21215-1-A, Proteintech), Notch1 (T55244S, Abmart; 20687-1-AP, Proteintech), Hes1(11988S, CST) and β-actin (ab179467; Abcam). .. Followed by secondary antibodies for 1 h. The PVDF membranes were detected using a chemiluminescence imager (Bio-Rad) and quantified using the Image Lab software (Version 4.1, Bio-Rad).

    Article Title: Time-dependent Lactiplantibacillus plantarum (LP) AS21 intervention enhances mesalazine efficacy by modulating gut microbiota and host immunometabolic responses in DSS-induced colitis.
    Article Snippet: .. The sections were then incubated overnight at 4 °C with 273 specific primary antibodies against occludin and claudin-1 (Proteintech Group, 274 China). ..



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